Green gram (Vigna radiata L.) is an important short-duration pulse crop cultivated widely in India, but its productivity is affected by several fungal diseases, including dry root rot caused by Macrophomina phaseolina (Tassi) Goid. The present study was undertaken to evaluate the in-vitro antifungal activity of natural farming inputs against M. phaseolina. The laboratory experiment was conducted in the Department of Plant Pathology, College of Agriculture, VNMKV, Parbhani. Seven natural farming inputs, viz., Ghan Jeevamrit, Jeevamrit, Neem cake, Beejamrit, Cow urine, fermented buttermilk and Ramban, were evaluated at 20 per cent concentration using the poisoned food technique. The experiment was conducted in a Completely Randomized Design with three replications, and radial mycelial growth was recorded on the seventh day after incubation. Considerable variation was observed among the treatments in their ability to suppress the mycelial growth of M. phaseolina. Beejamrit recorded the minimum colony diameter of 9.87 mm and the maximum mycelial growth inhibition of 89.04 per cent, followed by Jeevamrit, which recorded 24.17 mm colony diameter and 73.15 per cent inhibition. Cow urine recorded 46.27 mm colony diameter with 48.59 per cent inhibition, followed by Neem cake with 58.67 mm growth and 34.81 per cent inhibition. Ghan Jeevamrit and Ramban recorded 62.33 and 68.77 mm colony diameter, corresponding to 30.74 and 23.59 per cent inhibition, respectively. Fermented buttermilk showed the lowest inhibitory effect among the natural farming inputs, with 75.33 mm colony diameter and 16.30 per cent inhibition, whereas the untreated control recorded 90.00 mm radial growth. The findings demonstrated considerable differences in the antifungal activity of the tested natural farming inputs, with Beejamrit and Jeevamrit showing greater suppression of M. phaseolina under in-vitro conditions.