The variability studies indicated that the high estimate of phenotypic coefficient of variation (PCV) and genotypic coefficient of variation (GCV) was observed for seed cotton yield per plant. High phenotypic coefficient of variation and moderate genotypic coefficient of variation was observed for the number of monopodia per plant. High heritability observed for the plant height, upper half mean length, fiber strength, and uniformity ratio, the number of bolls per plant, micronaire and seed cotton yield per plant. The plant height, the number of bolls per plant and seed cotton yield per plant shows high heritability coupled with high genetic advance over mean indicating the preponderance of additive gene action in the inheritance of these traits.Genetic variability parameters were studied in forty-two cotton genotypes raised during kharif 2025-2026 at Cotton Research Station, Mahboob Baugh Farm, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani in randomised block design with two replications. The high degree of variability observed among genotypes for different yield contributing traits could be utilized in the breeding program for the improvement of desi cotton.