Macrophomina phaseolina is a destructive soilborne pathogen causing dry root rot in groundnut, and its variability has important implications for disease management. The present study was conducted to assess the cultural variability of M. phaseolina isolates associated with dry root rot in groundnut and to determine the influence of different solid media on mycelial growth and sclerotial production. Cultural studies were carried out on Potato Dextrose Agar, Czapek’s Dox Agar, Oatmeal Agar, and Richards Agar. The results revealed that the mycelial growth, sclerotia initiation and sclerotial production on all the media tested showed significant variations among the M. phaseolina isolates. Potato Dextrose Agar supported the maximum mycelial growth and sclerotial production, followed by Oatmeal Agar and Czapek’s Dox Agar, whereas Richards Agar recorded comparatively poor growth.