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VOL. 8, ISSUE 2 (2026)
Effect of different plant growth regulators on somatic embryogenesis in Prosopis cineraria (L.) Druce
Authors
Shweta Singhal, Anupama Goyal
Abstract
The present study investigates the influence of different plant growth
regulators (PGRs) on somatic embryogenesis in Prosopis cineraria, an
ecologically significant tree species of arid regions. A two-phase culture
system was employed to evaluate the role of auxins and cytokinins in
embryogenic callus induction and embryo development. High auxin concentrations,
particularly 2,4-dichlorophenoxyacetic acid (2,4-D) at 2 mg/L, were found to be
effective in inducing embryogenic callus, while subsequent transfer to media
containing reduced auxin or elevated cytokinin levels (0.5 mg/L NAA and 3 mg/L
BAP) promoted embryo differentiation and maturation. Leaf-derived callus
exhibited the highest embryogenic potential compared to other explants. Somatic
embryos developed through distinct stages and showed a high germination rate
(~75%), indicating their physiological competence. The findings highlight the
importance of hormonal balance and sequential media manipulation in regulating
somatic embryogenesis and provide an efficient protocol for large-scale
propagation and conservation of P. cineraria.
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Pages:39-41
How to cite this article:
Shweta Singhal, Anupama Goyal "Effect of different plant growth regulators on somatic embryogenesis in <i>Prosopis cineraria</i> (L.) Druce". International Journal of Agriculture and Plant Science, Vol 8, Issue 2, 2026, Pages 39-41
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