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International Journal of
Agriculture and Plant Science
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VOL. 8, ISSUE 1 (2026)
Bioactive effect of lime and lemon peels extracts on soil microflora load and disease incidence on soybean
Authors
Chimankpa W Worlu, Joy Worukwo-Emma Nneji, Nyekachi B Wofu, Fortune N C Okogbule
Abstract
This study investigated the bioactive effects of lime and lemon peels extracts on microbial load and disease incidence of soybean plant. The research was carried out at the demonstration site of Plant Science and Biotechnology, Rivers State University. Completely randomized design was adopted. Treatments were lime peel extract, lemon peel extract, and a combination of both at 5mgl-1, 10mgl-1 and 15mgl-1 concentration levels in triplicates plus the control. Colony forming unit was used to determine the soil microbial load 7 Days After Planting (7DAP) and visual assessment method was used to describe and estimate the quantitative damage that manifested in plant leaves 8 Weeks After Planting (8WAP) for the various concentrations. Results showed that lime and lemon peels extracts exhibited bioactive effect on the soil-borne fungal pathogens associated with soybean by reducing Mucor sp, Rhizopus sp, Aspergillus flavus, Penicillium notatum, Fusarium sp, Candida sp. load in the soil thereby inhibiting disease incidences such as chlorosis, leaf spot, leaf blight, rust and curling to economic manageable level. Mucor sp was mostly inhibited. The disease symptoms were susceptible to lemon and lime peels extracts at lower concentrations but had more inhibitory impact at higher concentrations. The combination of lime and lemon peels extracts showed rapid and higher antimicrobial activity as it relates to the overall disease manifestation on the vegetative and foliar parts of soybean. The extracts reduced the soil microbial loads and inhibited the foliar diseases to an economically manageable level and can be used for sustainable and eco-friendly agricultural practices.
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Pages:133-138
How to cite this article:
Chimankpa W Worlu, Joy Worukwo-Emma Nneji, Nyekachi B Wofu, Fortune N C Okogbule "Bioactive effect of lime and lemon peels extracts on soil microflora load and disease incidence on soybean". International Journal of Agriculture and Plant Science, Vol 8, Issue 1, 2026, Pages 133-138
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