A field experiment was conducted with eight fenugreek genotypes comprised of advanced lines and released varieties viz. UM-71, UM-68, UM-78, UM-80, UM-90, Rmt-305, Rmt-354 and Rmt-143 to assess the genetic variability under drought condition. Moisture stress was created by withholding irrigation in one set of genotypes at active growth stages while other sets were given normal irrigation. Different physiological, biochemical and antioxidant parameters, yield and yield contributing parameters were recorded under optimal and limited irrigation at flowering and pod filling stages. The result showed that different physiological parameters such as relative water content (RWC), chlorophyll content, carotenoids and membrane stability index decreased while biochemical parameters like proline, glycine betaine content and antioxidant enzymes like superoxide dismutase (SOD) and catalase increased under drought condition.