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VOL. 8, ISSUE 1 (2026)
Biochemical and phytochemical decoding of the leaf bioactive compounds in Giloy (Tinospora cordifolia) through GC QTOF-Ms/Ms and GC MS
Authors
Umamaheshwar Y, P J Rathod, Rukamsingh Tomar, Vala Ashishkumar, U K Kandoliya
Abstract
Tinospora cordifolia commonly
known as Giloy is a valuable medicinal plant used in Ayurvedic system of
medicine by Ancient Hindu physicians the experiments
conducted in Saurashtra region with decoding of phytochemicals presence in,
three crude extracts (methanol, ethanol and hexane) prepared from the leaf
tissue of Tinospora cordifolia also analysed through GC MS and GC Q-TOF MS MS. The results revealed the
significant variation in solubility of compounds in different extracts as well
as in number of compounds detected through high through put techniques. As
experimental data suggested highest phytochemical in GC-MS followed by GC-
QTOFs. It was found to be 127 compounds in GC MS profiling and remaining were
ranges between 6 to 7 in GC-QTOF MS/MS analysis. The linearity of this peaks
showed wider variabity in same compounds too. The higher amount of these
compounds in methanolic extracts namely, Eugenol,
7,9-Di-tert-butyl-1-oxaspiro (4,5) deca-6,9-diene-2,8-dione, Hexadecenoic acid, Pentanoic acid, 5-hydroxy-,
2,4-di-t-butylphenyl esters, 1,5-Cyclodecadiene,
1,5-dimethyl-8-(1-methylethenyl). as well as hexane leaf extract of Tinospora
cordifolia indicated the presence of 127 peaks and compounds. Out of them
94 are unique compounds. Methanolic extracts so wider variability in both the
platform. Phytochemical screening tests revealed ethanolic extracts is best
suitable for this plant. The biochemical composition showed that Moisture
(63.16%), Total carbohydrates (20.7%), protein (3.98). While the total phenol
was found 4.83% in leaf with antioxidants activity were shown to be 77.53% in Tinospora cordifolia.
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Pages:84-93
How to cite this article:
Umamaheshwar Y, P J Rathod, Rukamsingh Tomar, Vala Ashishkumar, U K Kandoliya "Biochemical and phytochemical decoding of the leaf bioactive compounds in Giloy (<i>Tinospora cordifolia) </i>through GC QTOF-Ms/Ms and GC MS". International Journal of Agriculture and Plant Science, Vol 8, Issue 1, 2026, Pages 84-93
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