Projects have been developed to enhance crops and establish a successful method for plant regeneration. This study focused on the regeneration response of cotyledonary node explants from germinating peanut (Arachis hypogaea L.) seeds. Sectioned cotyledonary nodes were grown in vitro to regenerate whole plants. Mixing 6-benzylaminopurine (BAP) with Murashige and Skoog's media yielded many shoots (1–50mg.L^-1), with optimal production for most varieties at 15 mg L^-1. Higher BAP concentrations inhibited all genotypes, while lower doses promoted repeated shoot production. Genotypic differences affected shooting potential with BAP presence. Two genotypes showed rooting on MS medium with 1 mg L^-1 NAA, whereas GJG 20 required a combination of 1 mg L^-1 NAA and 0.5 mg L^-1 IBA. Rooted plantlets were acclimatized in plastic cups with a soil, sand, vermicompost, and farmyard manure mix (3:1:1 w/w) for 7-10 days before field transfer. These plants flowered and produced healthy seeds without phenotypic variations, demonstrating a reliable in vitro regeneration method for peanut plants.