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VOL. 7, ISSUE 1 (2025)
Evaluation of SRAP marker efficiency to identifying genetic diversity among genotypes of greengram (Vigna radiata) (L.)
Authors
Manoj Parakhia, Zarna Vora, Neha Chovatiya, Hiren Bhalani, Shradha B Bhatt
Abstract
The genetic variability and relationships
among 12 greengram were analyzed using 121 sequencerelated amplified
polymorphism (SRAP) markers. 67 out of 121 SRAP primers were found to be the
most effective in fingerprinting the greengram genotypes under the study. Out
of 362 amplified bands, a total of 307 polymorphic bands were detected which
demonstrated with polymorphism information content (PIC) value of 0.695 with a
range from 0.142 to 0.906. The distinctive value of SRAP primer index (SPI) for
studied markers was 4.149. The unweighted-pair group method arithmetic average
(UPGMA) based grouping showed three distinct clusters at a cut-off value of
0.56. In the present study, the average Jaccard similarity coefficient was
0.561 which indicated a moderate level of genetic diversity in studied
greengram genotypes. The result of current investigation suggested that there
is need to increase the genetic base of greengram germplasm using different
breeding approach viz, mutagenesis, germplasm introduction, wide
hybridization or somaclonal variation.
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Pages:63-68
How to cite this article:
Manoj Parakhia, Zarna Vora, Neha Chovatiya, Hiren Bhalani, Shradha B Bhatt "Evaluation of SRAP marker efficiency to identifying genetic diversity among genotypes of greengram (<i>Vigna radiata</i>) (L.)". International Journal of Agriculture and Plant Science, Vol 7, Issue 1, 2025, Pages 63-68
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