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VOL. 6, ISSUE 1 (2024)
Assessment of the effectiveness of Metarhizium anisopliae and Beauveria bassiana in controlling Scrobipalpa ocellatella (Boh.), A damaging insect that affects sugar beet crops
Authors
Abla F A Saad, Abdel Raheem M A, Hala M Metwally, Eman M Elgohary
Abstract
Sugar beet (Beta vulgaris) is an important
crop in Egypt, contributing significantly to the economy. To meet the local
demand for sugar production, farmers are encouraged to increase their sugar
beet cultivation. However, the presence of the pest Scrobipalpa ocellatella
poses a threat to sugar beet plantations worldwide, leading to economic losses.
In order to obtain fungal isolates (Beauveria bassiana and Metarhizium
anisopliae), spores were collected by rinsing them with sterilized 0.5%
Tween 80 after 14 days the ancient culture (PDA) media was cultivated at a
temperature of 25±2ºC. Then, the spores of B. bassiana and M.
anisopliae were utilized to treat the fourth instar larvae and pupae of S.
ocellatella, using concentrations of 1x106, 1x107, and 1x108
spores/ml. After 9 days of treatment, the larvae exhibited a mortality rate of
100% when exposed to a concentration of 1x108 spores/ml of B.
bassiana. On the other hand, the same concentration of M. anisopliae
resulted in 100% mortality after 10 days. B. bassiana demonstrated
higher virulence against the pupae of S. ocellatella in comparison to M.
anisopliae. The LC50 value for infected larvae was 2.33x106
for M. anisopliae and 0.45x106 for B. bassiana, while
for infected pupae it was 1.98x107 and 2.97x106,
respectively.
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Pages:5-8
How to cite this article:
Abla F A Saad, Abdel Raheem M A, Hala M Metwally, Eman M Elgohary "Assessment of the effectiveness of <i>Metarhizium anisopliae </i>and <i>Beauveria bassiana</i> in controlling <i>Scrobipalpa ocellatella</i> (Boh.), A damaging insect that affects sugar beet crops". International Journal of Agriculture and Plant Science, Vol 6, Issue 1, 2024, Pages 5-8
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