This study was carried out to evaluation sensitivity of some tomato varieties to the insect infestation by Tomato leaf miners, Tuta absoluta (Meyrick). Experiments were carried out on five varieties of tomato, Solanum lycopersicum (Solanales: Solanaceae); Floraded, Preged, Mader, Beto86 and Alex63 at two locations (governorates), Perkash (Giza Governorate) and Tokh (Qaliobya Governorate) during season 2021 under plastic greenhouses. The study was carried out throughout three steps: first step includes studied the population fluctuations of T. absoluta on the successive tomato varieties at both of the two successive locations. Second step includes determination concentration of the most internal components of the successive tomato varieties such as (total proteins, total carbohydrates, total phenols and total lipids). And the third step includes determination the physiological changes in the successive insect T. absoluta throughout determination the changes in the important internal substances secreted by the insect such as total proteins, total carbohydrates, total lipids and the important enzymes such as: Chitinase, Lipase, Phosphatase, Kinase, Alpha esterase, Beta esterase, Oxidation enzymes and Digestive enzymes. Data obtained showed that the successive tomato varieties were arranged ascending in terms of the infestation by the successive insect T. absoluta at both of the two successive locations as follow: Floraded, Preged, Mader, Beto86 and Alex63 respectively. Data obtained showed also that concentration of the most internal components of the successive tomato varieties such as: total proteins, total carbohydrates, total phenols, total amino acids, tannins and flavonoids were arranged descending in the successive tomato varieties as follow: Floraded, Preged, Mader, Beto86 and Alex63 respectively. Lastly, data obtained showed that concentration of the internal components which secreted by the successive insect T. absoluta in insects which fed on the successive tomato varieties were arranged ascending as follow: Floraded, Preged, Mader, Beto86 and Alex63 respectively.