Biofertilizers are obtaining significant importance in sustainable agricultural trends because of their environment-friendly nature, ability to increase soil fertility and health, producing chemical-free products. The hazardous effect of synthetic fertilizers on human health and the environment, high cost, etc. are leading to choose alternative options of farming. Administration of biofertilizers enhances crop productivity, improves soil fertility and maintains an eco-friendly environment as well. The present study is focused to evaluate the effect of the application of liquid biofertilizers viz., PSB, KMB, and KSB isolate on growth, yield, and nutrient content uptake in lettuce under greenhouse conditions. Individual and co-inoculation of PSB, KMB, and KSB isolates have highly influenced the biomass, number of leaves as well as nutrient content of lettuce. The experiment was conducted in randomized block design containing six treatments both individual and in consortia. The growth parameters evaluated were root length, shoot length, number of leaves, fresh weight, and dry weight. Among the six treatments, co-inoculation with PSB+KMB+KSB (T4) recorded the highest root length, shoot length, number of leaves, fresh weight, and dry weight. All the other treatments were par with each other, whereas, control (uninoculated) recorded the least.