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VOL. 4, ISSUE 2 (2022)
Isolation and morphological characterization of rice pathogens Pyricularia and Bipolaris Oryzae in metekal zone, north west Ethiopia
Authors
Tesfaye Gudisa Waktola
Abstract
Rice blast disease, caused by Pyricularia oryzae Cavara is one of the major biotic factors and the most destructive of all rice diseases impeding rice production in more than 85 countries in the world. Brown spot disease of rice is caused by Bipolaris oryzae (Breda de Haan) Shoemaker, (Teleomorph – Cochliobolus miyabeanus) was major problem eventually caused sustainable losses both in quality and quantity. The present study aimed to characterize morphologically rice blast and brown spot diseases. Zones and districts were selected purposely based on rice area coverage at heading to dough crop growth stage. The samples were collected from 72 farmers’ fields of rice and six localities in each two districts. It was conducted during mid-October to mid November 2020/2021 main cropping season in Pawe and Jawi districts. The fungus, P. oryzae and B. oryzae were isolated from single discrete lesion from the infected leaf tissue or panicle. Commercial potato dextrose agar (PDA) medium was used for isolation. Bacterial growth/contamination was avoided by incorporating antibiotic chloramphenicol in the media. The growing mycelium were purified and replicated two times on PDA by exploiting the principle of completed randomized design (CRD). The isolates were characterized based on the morphological characteristics of the pathogen with the help of keys by Barnett and Hunter. The results revealed that, there was a considerable significant variation among the isolates of P. oryzae and B. oryzae in colony radial growth, conidial length and conidial width on PDA media. From the total of 45 P. oryzae and 32 B. oryzae isolates, 15 and 11 isolates, respectively were shown morphological variations.
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Pages:8-15
How to cite this article:
Tesfaye Gudisa Waktola "Isolation and morphological characterization of rice pathogens <em>Pyricularia</em> and <em>Bipolaris</em> <em>Oryzae </em>in metekal zone, north west Ethiopia". International Journal of Agriculture and Plant Science, Vol 4, Issue 2, 2022, Pages 8-15
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