Poaceae family is considered to be stenopalynous as all the member exhibit similar pollen morphology being monoporate and psilate (Sometimes microreticulate). However, the occurrence of diporate pollen grains which is a rare phenomenon in the family, has been reported by various authors in recent times from different parts of the world, occurring in few species. Palynomorphological studies of Poaceae in India are quite meagre and the report of diporate pollen grains in Indian Poaceae is scanty. The present study deals with 19 species of Indian Poaceae to investigate the presence of diporate pollen grains. Freshly collected pollen grains were acetolysed and studied under bright field light microscopy and their respective microphotographs were taken. The study reveals the existence of diporate pollen grains in one of the members i.e. Paspalidium flavidum, which is not reported in earlier studies. Paspalidium flavidum exhibits both the type of pollen morphs, i.e. monoporate as well as diporate but monoporate pollen grains are dominant. It can serve as a taxonomic tool for identification of the species and it can be assumed that the increase in the number of apertures in Poaceae members could be an evolutionary phenomenon with an effect of environmental condition acting on it.