Sugar industry effluent is a very important agro-based industry in India and discharges large amount of into water bodies which affects the plants and other living organism. In the present investigation, the physio-chemical analysis of sugar industry effluent was determined and the impact of different concentrations of [control 0, 25, 50, 75, 100 %] sugar mill effluent on seed germination behaviour of Moth bean (Vigna aconitifolia) was studied. In addition, we also analysed the morphological parameters such as shoot length, root length, fresh and dry weight, photosynthetic pigments and biochemical compounds were calculated. The results recorded for the analysis of sugar mill effluent indicated some parameters such as pH, EC, sulphate, copper, potassium, sodium, chlorine exceeded the permissible limit compared to TNPCB (Tamil Nadu Pollution Control Board). The morphological parameters, photosynthetic pigments and biochemical compounds increased in lower concentration of sugar mill effluent. Thus lower concentration of effluent may be used as a liquid fertilizer for agricultural purpose