Copper Sulphate treatment produced toxic effects on seed germination and seedling growth of Soyabean crop along with significant reduction in seedling dry weight as compared to control treatment. Similarly, the tolerance to copper treatment decreased the tolerance indices for water Soyabean seedlings with the increase in metal concentration in the substrate as compared to control. The difference in tolerance and seedling vigour index in response to copper sulphate toxicity should be considered while Soyabean cultivated in copper contaminated areas. Seeds treated with distilled water were recorded highest seed germination percentages and also root, shoot, seedling length and dry weight of seedling, followed by seeds treated with copper sulphate, decreases the overall growth parameter of seedling.